Supplementary Figure 3: Baseline vessel diameters, RBC flow velocity and thickness of the arteriole smooth muscle cell layer in 1- to 2-month-old pericyte-deficient Pdgfrb+/− mice and age-matched littermate controls.
From: Pericyte degeneration leads to neurovascular uncoupling and limits oxygen supply to brain
(a) Average baseline in vivo vessel diameters (mean ± 95% CI) prior to stimulation determined for 21 total arterioles from 9 Pdgfrb+/+ mice, 27 total arterioles from 11 Pdgfrb+/− mice, 57 total capillaries from 10 Pdgfrb+/+ mice and 40 total capillaries from 10 Pdgfrb+/− mice (arterioles: t-test, equal variance: t = 0.27, p = 0.79; capillaries: t-test, equal variance: t = 1.04, p = 0.31). (b) Average (mean ± 95% CI) baseline in vivo RBC velocity acquired prior to stimulation in 14 total arterioles from 6 Pdgfrb+/+ mice and 23 total arterioles from 10 Pdgfrb+/− mice, and 32 total capillaries from 9 Pdgfrb+/+ mice and 36 total capillaries from 12 Pdgfrb+/− mice (t-test, equal variance: arterioles: t = 0.23 p = 0.82, capillaries: t = 0.33, p = 0.74). (c) Representative images of the smooth muscle cell layer (SMα, red) thickness and lack of pericyte coverage (CD13, magenta) on arterioles. (d) Thickness of the arteriolar smooth muscle cell layer determined by confocal microscopy analysis. Mean ± 95% CI from 42 arterioles from 7 Pdgfrb+/+ mice and 45 arterioles from 6 Pdgfrb+/− mice (t-test, equal variance: t = 1.14, p = 0.28).Two-tail t-tests used.
