Supplementary Figure 4: CRFR1PVN-cKO mice and control mice display similar plasma CORT levels following acute stressors.
From: Hypothalamic CRFR1 is essential for HPA axis regulation following chronic stress
CRFR1PVN-cKO mice and control mice display similar plasma CORT levels following acute stressors. CORT radioimmunoassay was performed in order to measure the circulating CORT levels, following exposure to restraint stress (a), hemorrhage (b), LPS injection (c) and DEX suppression test in which blood was collected 4 hrs after DEX administration (1 μg/10 g BW i.p.) (d). CRFR1PVN-cKO mice did not differ from control mice in any of the tests [based on repeated measure ANOVA (a; F(1,10)=0.28, n.s. b; F(1,15)=0.11, n.s. and c; F(1,12)=1.13, n.s.) and independent sample t-tests (d; t(10)=-1.32, n.s. and t(10)=-0.97, n.s.) before and after DEX administration, respectively. n=11 control mice and 9 CRFR1PVN-cKO mice (a), n=9 mice (b), n=8 (c) and n=7 control mice and 5 CRFR1PVN-cKO mice (d)]. Data presented as mean ± SEM.
