Supplementary Figure 5: Motif prediction of neuronal activity-induced gained-open and gained-closed sites at E1 compared to E0 by two different algorithms, HOMER and MEME-ChIP.

(a) De novo motif identified by MEME-ChIP analysis of neuronal activity-induced gained-open regions at E1 resembles AP-1 complex, including cFos, JunB, Jun and JunD. Refer to Fig. 3a. (b) De novo motif identified by MEME-ChIP analysis of neuronal activation-induced gained-closed regions at E1 does not recover motifs similar to known transcription factor binding motifs (P = 4.5e -51). (c) De novo motif identified by HOMER from neuronal activity-induced gained-open and gain-closed sites at E1. ChIP-seq data for cFos, FosB, JunB and CREB used for plots were from a published dataset⁷