Supplementary Figure 6: Characterization of c-Fos expression in the adult mouse dentate gyrus.
From: Neuronal activity modifies the chromatin accessibility landscape in the adult brain
(a, b) Expression of endogenous cFos in response to transient synchronous neuronal stimulation. Shown are summaries of time-course analyses of mRNA (a) and protein (b) expression of cFos in the adult dentate gyrus after electroconvulsive stimulation. Values represent mean ± s.e.m. (n = 3 for a, n = 2 for b; *P < 0.05; **P < 0.01; ANOVA). Full-length western blots are presented in Supplementary Fig. 10. (c) Schematic diagrams of AAV constructs used to express shRNA or exogenous protein in the adult mouse dentate gyrus (top panel) and the experimental paradigm (bottom panel). (d, e) Q-PCR analysis of expression of cFos in the adult dentate gyrus expressing shRNA-Ctrl and shRNA-cFos (d) or cFos and/or EYFP (e). Values represent mean ± s.e.m. (n = 3 mice; *P < 0.05; permutation test).
