Supplementary Figure 6: Optical stimulation and electrophysiological recordings in the mPFC of mice expressing ChR2-eYFP and eYFP alone.
From: One-step optogenetics with multifunctional flexible polymer fibers
(a) Neural recordings collected with a fiber probe implanted in the mPFC. The same fiber was used to deliver AAV5-CamKIIα::ChR2-eYFP 14 days prior to the recording. Spontaneous activity is observed prior to the first optical stimulation epoch (10 Hz, 5 ms pulse width, 6.4 mW/mm2). Stimulation epoch is followed by depletion and recovery, which may be attributed to local depletion of neurotransmitters. (b, c) Electrophysiological recordings during optical stimulation in the mPFC using a multifunctional fiber probe (b) 3 days, (c) 2 weeks following transfection with AAV5-CaMKIIα::eYFP. There were no light-evoked signal for both case.
