Supplementary Figure 4: Optogenetic interrogation in the mouse mPFC with the multifunctional fiber probes.
From: One-step optogenetics with multifunctional flexible polymer fibers
(a, b) Expression of AAV5-CamKIIα::ChR2-eYFP in mPFC following delivery through a multifunctional fiber probe. The expression was observed in a wild-type mouse 2 weeks following viral injection. The size of transfected region was defined by the amount of injected virus solution. (a) 0.1 μl and (b) 0.5 μl of virus. Scale bar = 1 mm. (c) Dependence of the amplitude of optically-evoked potentials on the stimulation power density in the mPFC 2 weeks, 1 month, and 3 months following injection of AAV5-CamKIIα::ChR2-eYFP (Number of animals n=8). All electrophysiological recordings, optical stimulation and viral delivery were performed through the multifunctional fiber probes. Error bars represent standard deviation.
