Supplementary Figure 3: Differential propagation of dendritic excitation to the soma in sPCs and cPCs revealed by TREES and NEURON simulations.
From: A distinct entorhinal cortex to hippocampal CA1 direct circuit for olfactory associative learning
Simulation was performed under either (a−c) MATLAB TREES toolbox or the (d−g) NEURON stimulation. (a) A pair of example sPC and cPC from experimental reconstruction showing dendritic sites of current injection (blue and red dots) and the recording at soma. (b) Example of somatic voltage change (ΔV, upper) elicited by injected electronic currents at ∼450 μm apical dendrite (bottom) in the sPC (blue) and cPC (red), respectively. (c) Cumulative plot of mean ΔV of 171 sPCs (blue) and 183 cPCs (red). Each mean ΔV for a given PC was averaged from all trials with the injection sites distributed on all distal dendritic sections (> 300 μm). Statistic difference was measured by two-sample Kolmpgorov-Smirnov test, P < 0.001. (d) Morphologies of sPC and cPC NERUON models selected from the ModelDB65,66 depicting distal synaptic inputs, dendritic and somatic recordings. (e,f) Same as b−c except using NEURON models. (g) Comparison of mean somatic and dendritic excitation between sPC and cPC. Data were presented as mean±s.e.m. Statistic difference was measured by unpaired t-test, P < 0.001 and P = 0.76 for the somatic and local dendritic injections, respectively.
