Supplementary Figure 8: Characterization of Calb2-IRES-Cre::Ai9 and Calb1-2A-dgCre::Ai9 mice.
From: A distinct entorhinal cortex to hippocampal CA1 direct circuit for olfactory associative learning
(a,b) Example images showing the genetically-tdTomato labeled cells (red) immunostained with monoclonal antibody against calbindin-D28k (green) in the dCA1 of (a) Calb2-IR ES-Cre::Ai9 and (b) Calb1-2A-dgCre::Ai9 (7-d TMP induction, see Methods) mice. Scale bar, 100 μm. (c,d) Characterization of hippocampal labeling from anterior (A) to posterior (P) in (c) Calb2-IR ES-Cre::Ai9 and (d) Calb1-2A-dgCre::Ai9 (7-d induction) mice. Scale bar, 1 mm. All following statistic results are measured in the sections from Bregma -2.0 to -3.5 mm with 0.3 mm step along the A−P axis. (e,f) Comparison of the labeling efficiency (yellow/green) and colocalization rate (yellow/red) in the (e) CA1 SP and (f) DG between the Calb2-IRES-Cre ::Ai9 and Calb1-2A-dgCre ::Ai9 (with 1 or 7 d induction) mice, respectively. (g,h) Fluorescence images showing the (g) neuronal GABA staining in the dCA1 of Calb2-IRES-Cre ::Ai9, and the (h) tdTomato labeling rates in GABAergic cells in different layers of dCA1. Note the co-localization rates are < 10%. Scale bar, 100 μm. (i,j) Similar as g,h except for Cre antibody staining. Note that Calb2-IRES-Cre ::Ai9 mice exhibit high efficiency (> 90%) in labeling neurons in the dCA1 SP layer, but low efficiency (< 15%) in labeling neurons in other layers. Scale bar, 100 μm. Data were presented as mean±s.e.m.
