Supplementary Figure 4: Identification of CCN3 as a mediator of Treg-induced oligodendrocyte differentiation
From: Regulatory T cells promote myelin regeneration in the central nervous system
(a) Semi-quantitative proteome profiler analysis of non-polarized (NP) and Treg-conditioned media (2 independent batches) compared to cell culture medium only. (b) Representative immunofluorescence images of mixed glial cultures stained for Olig2+MBP+ cells and analyzed quantitatively in (Figure 4d) (Green = Olig2, red = MBP, scale bar = 100 μm). (c) Schematic diagram of CCN3 depletion and elution from Treg-conditioned media. (d) ELISA confirmation of CCN3 depletion from Treg-conditioned media n = 2. Data representative of 1 (a, with 2 independently generated batches), or at least 3 (b, d) biologically independent experiments.
