Supplementary Figure 11: Motor behaviors are not rescued by Isx-9 treatment in R6/2 mice. | Nature Neuroscience

Supplementary Figure 11: Motor behaviors are not rescued by Isx-9 treatment in R6/2 mice.

From: Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice

Supplementary Figure 11

Mice were initially tested for specific HD related behavior deficits using tasks that examine motor impairment. Genotype effects were observed by 8 weeks; however, Isx9 did not provide statistically significant differences in treated versus control mice in rotarod (a) pole test (b) or grip strength (c) assays. (a) Rotarod indicates no motor improvements in Isx-9 treated mice. Non-transgenic (NT) and R6/2 mice treated with vehicle (veh) or Isx-9 were subjected to the rotarod task to evaluate motor ability. Impairment was observed in R6/2 mice. Points represent individual mouse scores of time on rotarod measured in seconds. Center lines represent mean of each groups data set and error bar whiskers are indicative of SEM (n=10/group at 8 weeks age). Statistical tests used one-way ANOVA followed by Tukey HSD Test with Scheffé, Bonferroni and Holm multiple comparison calculation also performed post hoc. One-way ANOVA F value 92.8 with 3 degrees of freedom, ***p=0.001. (b) Pole test indicates no motor improvements in Isx-9 treated R6/2 mice. Non-transgenic (NT) and R6/2 mice treated with vehicle (veh) or Isx-9 were subjected to the pole test task to evaluate motor ability. Impairment was observed in R6/2 mice. Points represent individual mouse scores of time to descend measured in seconds. Center lines represent mean of each groups data set and error bar whiskers are indicative of SEM (n=10/group at 9 weeks age). Statistical tests used one-way ANOVA followed by Tukey HSD Test with Scheffé, Bonferroni and Holm multiple comparison calculation also performed post hoc. One-way ANOVA F value 9.7 with 3 degrees of freedom, ***p=0.001. (c) Grip strength test indicates no improvements in Isx-9 treated mice. Non-transgenic (NT) and R6/2 mice treated with vehicle (veh) or Isx-9 were subjected to the grip strength task to evaluate forelimb grip. Impairment was observed in R6/2 mice. Points represent individual mouse scores of grip measured in grams of strength. Center lines represent mean of each groups data set and error bar whiskers are indicative of SEM (n=10/group at 10 weeks age). Statistical tests used one-way ANOVA followed by Tukey HSD Test with Scheffé, Bonferroni and Holm multiple comparison calculation also performed post hoc. One-way ANOVA F value 13.6 with 3 degrees of freedom, ***p=0.001.

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