Supplementary Figure 1: Staining and quantification of neuronal and oligodendrocyte differentiation markers. | Nature Neuroscience

Supplementary Figure 1: Staining and quantification of neuronal and oligodendrocyte differentiation markers.

From: Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice

Supplementary Figure 1

(a) Representative images of immunocytochemistry at day 56 of differentiation demonstrate that both HD (HD109 pictured here) and non-disease (CTR21 pictured here) iPSC lines can generate glial (GFAP) and neuronal (TUJ1, MAP2ab, DARPP32) cells. Scale bar represents 100 mm. (b) Non-biased stereological counts of TUJ1 at day 56 of differentiation indicate that there is no difference in the percent of cells TUJ1-positive between HD and non-disease (CTR). The HD109Q line did have significantly lower TUJ1-positive cells than the CTR21Q and the HD60Q lines (one-way ANOVA, * p<0.05, ** p<0.01); however, this reflects line-to-line variability versus a CAG repeat effect. (c) Non-biased stereological counts of MAP2ab at day 56 of differentiation indicate that there is no difference in the percent of cells MAP2ab-positive between HD and non-diseased. (d) Non-biased stereological counts of DARPP32 at day 56 of differentiation indicate that while there is no difference in the percent of DARPP32-positive cells between HD and non-disease. (e) Non-biased stereological counts of GFAP indicate there is no difference in the percent of cells GFAP-positive between HD and non-diseased. (f) Glial markers PDGFRα and O4 were found to be absent in the iPSC-neural cultures at day 60 of differentiation, whereas at day 112 of oligodendrocyte differentiation from iPSCs, these glial markers were found. Approximately 1000 cells were counted per slide for three independent differentiations.

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