Supplementary Figure 12: Whisker stimulation-evoked neural activity was unaffected by 100 μM Ba²⁺ superfusion.

(A) Representative 8 s LFP epoch (top) and accompanying 0-20 Hz spectrogram (bottom) recorded from the whisker barrel cortex in response to contralateral whisker stimulation under control conditions. Stimulation reliably entrained large oscillations at a frequency of ~5 Hz. (B) Exactly as in A for the same mouse, after superfusion of 100 μM Ba²⁺ over the cranial surface. (C) Signal spectrum plot for the recording in A illustrating the predominance of 5 Hz oscillations evoked by whisker stimulation. (D) Signal spectrum plot for the recording in B. (E) Summary data showing the peak signal amplitude before and after 100 μM Ba²⁺ superfusion for the 0 – 4 Hz band of the LFP spectrum (n = 5 paired experiments, 5 mice; P = 0.3778 (t₄ = 0.991) Student's paired t-test). (F) Summary data showing the frequency of peak LFP oscillations before and after 100 μM Ba²⁺ superfusion for the 0 – 4 Hz band of the LFP spectrum (n = 5 paired experiments, 5 mice; P = 0.4804 (t₄ = 0.777) Student's paired t-test). (G) Summary data showing the peak signal amplitude before and after 100 μM Ba²⁺ superfusion for the 4 – 6 Hz band of the LFP spectrum (n = 5 paired experiments, 5 mice; P = 0.5627 (t₄ = 0.6303) Student's paired t-test). (H) Summary data showing the frequency of peak LFP oscillations before and after 100 μM Ba²⁺superfusion for the 4 – 6 Hz band of the LFP spectrum (n = 5 paired experiments, 5 mice; P = 0.1202 (t₄ = 1.969) Student's paired t-test). (I) Summary data showing the peak signal amplitude before and after 100 μM Ba²⁺ superfusion for the 6 – 20 Hz band of the LFP spectrum (n = 5 paired experiments, 5 mice; P = 0.755 (t₄ = 0.3337) Student's paired t-test). Note that the scale is one order of magnitude smaller than for the data in other bands, reflecting lower activity in this frequency range. (J) Summary data showing the frequency of peak LFP oscillations before and after 100 μM Ba²⁺superfusion for the 6 – 20 Hz band of the LFP spectrum (n = 4 paired experiments, 4 mice; P = 0.907 (t₃ = 0.1233) Student's paired t-test). All error bars represent s.e.m.