Supplementary Figure 5: Criteria for identifying Aβ-, Aδ- and C-fiber-mediated synaptic inputs in the spinal dorsal horn

(a) Image is our sagittal spinal cord slice preparation with DRG and full length of dorsal root attached. Schematics illustrate how to measure distance from the tip of the stimulation electrode to the tip of the recording electrode (the distance is about 5 mm under our recording conditions), and how to perform dorsal root compound action potential recordings. (b) Summary of thresholds, conduction velocities, and shortest latencies for primary afferent Aβ, Aδ and C fibers determined by dorsal root compound AP recordings (from 9 mice). (c) Image is our sagittal spinal cord slice preparation with DRG and full length of dorsal root. Schematics illustrate how to measure distance from the tip of the stimulation electrode to the entrance of the attached dorsal root (the distance is about 8 mm under our recording condition), and the location of the suction electrode when we perform recordings in the spinal dorsal horn. (d) Typical traces illustrate high frequency following with constant latency to assess monosynaptic inputs or not. (e) Parameters for identifying Aβ/Aδ/C-fiber mediated synaptic inputs in the spinal dorsal horn.