Supplementary Figure 13: Nerve-injury-opened C-fiber inputs to VT3Cre-negative neurons in vIIi
From: Identification of spinal circuits involved in touch-evoked dynamic mechanical pain
(a) Schematic showing sagittal dorsal root-spinal cord slice preparation that removed low-threshold Aβ-fiber inputs to dorsal horn neurons. (b) No response to Aβ intensity stimulations in both naïve and SNI mice. (c) C intensity stimulations evoked EPSCs insufficient to fire APs in naïve mice (0/30) (see Fig. 7d), but fired APs in 5/10 neurons in SNI mice (χ2 test; P < 0.0001, χ2 = 17.1429), plus an electrically high-threshold Aβ input without AP firing. Blue and red arrows indicate C fiber inputs and electrically high threshold Aβ inputs/outputs (“HT-Aβ”), respectively. Typical traces shown are representative responses of 5 out of 10 VT3Cre-negative neurons recorded in vIIi. Black arrows indicate stimulation artifact, red vertical dashed lines indicate the 10-ms time point following stimulation. All recordings are composed of three repeated traces.
