Supplementary Figure 5: BLA and PL exhibited less correlated activity during a habituation session prior to the acquisition of the reward and fear memories, as well as during a neutral cue after acquiring the memories.
From: Amygdala inputs to prefrontal cortex guide behavior amid conflicting cues of reward and punishment
Related to Figure 2. (a) On a subset of animals (n = 4), BLA/PL recordings were performed during an initial habituation session in which three cues (light, tone, and white noise) were presented without any outcome. These cues were then paired with either sucrose, shock, or no outcome to become the CS-Suc, CS-Shock, and neutral CS–, respectively. Recordings were then performed during a discrimination session after animals acquired these associations. (b-c) Behavioral responses during the discrimination session. While animals showed selective port entry and freezing responses to the CS-Suc and CS-Shock, respectively, they did not display any of these behaviors during the neutral CS–. Error bands represent s.e.m. (d) Proportion of BLA/PL neural pairs that exhibited significantly correlated activity during either the habituation or discrimination session. Significantly higher proportions of BLA/PL neural pairs exhibited correlated activity during the discrimination session than the habituation session during all epochs, except the CS– (Bonferroni-corrected chi-square tests comparing habituation versus discrimination: ITI, X2 = 21.6, ***P < 0.001; CS-Suc, X2 = 18.6, ***P < 0.001; CS-Shock, X2 = 13.0, **P = 0.0012; CS–, X2 = 2.91, P = 0.36). In addition, during the discrimination session there were fewer correlated neural pairs during the CS– than during the other epochs (Bonferroni-corrected chi-square tests: CS– vs ITI, X2 = 8.40, *P = 0.015; CS– vs CS-Suc, X2 = 3.7, P = 0.22; CS– vs CS-Shock, X2 = 3.11, P = 0.31). These findings support the hypothesis that BLA/PL correlations strengthened with learning.
