Supplementary Figure 8: Histological assessment for the BLA→PL stimulation experiments and behavioral effects during a discrimination session with a neutral cue.
From: Amygdala inputs to prefrontal cortex guide behavior amid conflicting cues of reward and punishment
Related to Figure 6. (a) Histological reconstruction of viral infusions and location of optical fibers (eYFP: n = 10 animals; ChR2: n = 8 animals). Circles in the BLA drawings represent the center of viral infusions. Dashes in the PL drawings represent the optical fiber tips. (b) Histology for the pharmacology experiment (n = 10 animals). Dashes in the PL drawings represent the position of cannulas and optical fibers. (c) Photostimulation during the discrimination of a CS-Suc, CS-Shock, and a neutral cue (CS–). Animals were tested over two days shortly after infusion of either ACSF or NBQX+AP5. Difference scores are plotted relative to laser-OFF trials. A significant interaction between the drug and laser treatments was detected for freezing responses during CS-Shock trials (repeated measures two-way ANOVA with Bonferroni post-hoc tests: F1,18 = 7.57, P = 0.013; t18 = 3.89, ***P < 0.001). No significant interaction effects were detected for port entries during CS-Suc trials (F1,18 = 0.01, P = 0.97). No significant interaction effects were detected for either behavior during the CS– (freezing, F1,18 = 0.50, P = 0.49; port entry, F1,18 = 2.84, P = 0.11). Error bars represent s.e.m.
