Supplementary Figure 9: Validation of the ArchT viral construct and effects of optogenetically mediated inhibition of BLA inputs on the spontaneous firing of PL neurons.
From: Amygdala inputs to prefrontal cortex guide behavior amid conflicting cues of reward and punishment
Related to Figure 7. (a) Single-unit activity was monitored in the BLA at several time points after viral infusion to determine whether the ArchT construct produced reliable silencing. In the coronal drawings, four recording sites are illustrated per animal (n = 4 subjects), as microlesions were performed on four representative channels along the circumference of the optical fiber. (b-c) BLA units exhibiting ArchT-induced inhibition or excitation. (d) Quantification of the BLA cells that exhibited significant inhibition (“-”), excitation (“+”), or no change (“No Δ”). ArchT-induced inhibition predominated at all recording time points (chi-square tests comparing inhibited and excited populations: 10 days, X2 = 9.07, **P = 0.003; 20 days, X2 = 19.4, ***P < 0.001; 30 days, X2 = 20.0, ***P < 0.001). (e-f) Population histograms for the inhibited and excited BLA cells. (g) Assessment of spontaneous activity in PL upon local ArchT-induced inhibition of BLA inputs. Coronal drawings show the recording sites in PL. (h) Quantification of the PL cells that exhibited significant changes in activity during inhibition of BLA inputs. Inhibition of BLA inputs produced sparse effects on the spontaneous activity of PL neurons (Fisher exact probability tests: 10 days, P = 1.00; 20 days, P = 0.50; 30 days, P = 0.25). Error bands represent s.e.m.
