Supplementary Figure 4: Cells of the putative lymphatic loop express lymphatic markers and are separate from the blood vasculature.
From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish
(a) Confocal images of a 5dpf Tg(-5.2lyve1b:DsRed)nz101; Tg(kdrl:EGFP)s843 zebrafish midbrain showing that cells of the lymphatic loop do not co-express lyve1 and the blood vessel marker kdrl. n=5 embryos. Full quantification of marker co-expression can be found in Supplementary Figure 1f.
(b) Confocal images of lyve1/prox1a positive cells of the lymphatic loop at 5dpf in Tg(-5.2lyve1b:DsRed)nz101; Tg(prox1a:KalTA4uq3bh;10xUAS:Venus) zebrafish. n=3 embryos. Full quantification of marker co-expression can be found in Supplementary Figure 1f.
(c) Confocal images of a 5dpf zebrafish head showing cells of the lymphatic loop co-express the lymphatic markers lyve1 and fli1a in Tg(-5.2lyve1b:DsRed)nz101; Tg(fli1a:EGFP) (arrowheads). n=5 embryos. Full quantification of marker co-expression can be found in Supplementary Figure 1f.
(d) Confocal images of 5dpf Tg(-5.2lyve1b:DsRed)nz101 embryo injected with dextran into the blood vasculature. The lyve1 positive cells do not contain dextran demonstrating they are separate from the blood vasculature. Scale bars represent 100μm. Representative of analysis from n=4 embryos.
