Supplementary Figure 5: Kaede photoconversion demonstrates that the lymphatic loop gives rise to the meningeal muLEC population.
From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish
(a-b) Confocal images of Tg(prox1a:KalTA4)uq3bh; Tg(10xUAS:Kaede)s1999t photoconverted at 10dpf and reimaged at 15dpf (a) and photoconverted at 20dpf and reimaged at 25dpf (b). Scale bars represent 50μm. For the 10-15 dpf stages n=34 cells from 7 larvae were examined and for the 20-25dpf stages n=29 cells from 3 larvae were analysed. Positive lineage tracing was confirmed in all cases. (c) Quantification of the average increase in photo-converted cell number over 5 days (proliferation rate, cell number 5 days after conversion/0 days after conversion) data combined for 5-20 dpf experiments, n=19 larvae overall. Error bars represent mean +/− sem, Statistical testing N/A.
