Supplementary Figure 9: muLECs can be FAC-sorted and separated from blood endothelial cells and pericytes. | Nature Neuroscience

Supplementary Figure 9: muLECs can be FAC-sorted and separated from blood endothelial cells and pericytes.

From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish

Supplementary Figure 9

(a) Representative plots of FAC sorted cells from adult brains in unlabeled wildtype and Tg(-5.2lyve1b:DsRed)nz101 positive samples.

(b) Representative plots of FAC sorted EGFP positive cells in unlabeled wildtype and Tg(-5.2lyve1b:DsRed)nz101; Tg(kdrl:EGFP)s843 positive samples.

(c) Representative plots of FAC sorted EGFP positive cells in unlabeled wildtype and Tg(-5.2lyve1b:DsRed)nz101; TgBAC(pdgfrβ:EGFP)uq15bh positive samples.

(d) Scree plot of variance (eigenvalue versus component number) and principle component comparisons from Fig. 5b shown in 2-dimensional plots (PC1 vs PC2, PC2 vs PC3 and PC1 vs PC3), clear separation of muLECs from control samples is observed.

(e) Representative plots of FAC sorted EGFP positive cells in wildtype and Tg(pdgfrβBAC:EGFP)uq15bh; Tg(kdrl:mCherry)s843 positive samples.

Back to article page