Supplementary Figure 11: Visual confirmation of muLEC ablation and mock ablation.
From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish
(a) Single z-slices from a dorsal confocal image of a 10dpf Tg(-5.2lyve1b:DsRed)nz101;Tg(fli1a:nEGFP)y7 larvae before and after targeted ablation of muLECs. Ablation can be confirmed by direct observation of cell death, with “bubbles” which disperse post ablation. Scale bar represents 50μm. Representative images of n=27 larvae analysed.
(b) Second example of single z-slices from a dorsal confocal image of 10dpf Tg(-5.2lyve1b:DsRed)nz101;Tg(fli1a:nEGFP)y7 larvae before and after ablation of muLECs. Ablation can be confirmed by direct observation of cell death. The analysis in Fig. 7 used the same approach to verify cell ablations for n=27 embryos in total.
(c) Example of single z-slices from a dorsal confocal image of 10dpf Tg(-5.2lyve1b:DsRed)nz101;Tg(fli1a:nEGFP)y7 larvae before and after mock ablation in which cells adjacent to the muLECs are targeted. Ablation confirmed by direct observation and this approach was used in Fig. 7 for analysis of n=15 larvae. This is the same larvae as in Fig. 7g,h. Scale bar represents 50μm.
(d) Second example of single z-slices from a dorsal confocal image of 10dpf Tg(-5.2lyve1b:DsRed)nz101;Tg(fli1a:nEGFP)y7 larvae before and after mock ablation in which cells adjacent to the muLECs are targeted. This is the same larvae as above and in Fig. 7g,h.
(e and f) 10 dpf Tg(-5.2lyve1b:DsRed)nz101;Tg(fli1a:nEGFP)y7 larvae before (e) and after (f) mock ablation. Representative of n=15 larvae analysed. Scale bar represents 100μm.
