Supplementary Figure 12: Schematic summary of muLEC development and function
From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish
a. Mural lymphatic endothelial cells (muLECs) emerge from the choroidal vascular plexus (CVP) at 54 hpf (a) and sprout to the periphery of the midbrain at 96 hpf (a’).
b. The resulting lymphatic vascular loop in the midbrain (MB) begins to undergo a transition to a mesenchymal morphology between 5 (b’) and 10 dpf (b’’)
c - d. muLECs (blue) are present on the midbrain, fore brain (FB) and hind brain (HB) in 7 mm larvae (c) and expand over the adult brain (d). c’ and d’ provide lateral views and indicate the meningeal lymphatics (green, c’) and muLEC location relative to blood vessels (red) (d’).
e and f. Three dimensional (e) and cross sectional schematic representation of the blood vasculature and mural cells at the zebrafish meninx. muLECs (blue) secrete vascular growth factors and take up LDL from the bloodstream. PVM= perivascular macrophage.
