Supplementary Figure 2: High-molecular-weight dye can be taken up by meningeal lymphatics but not by muLECs.
From: Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish
(a) Dorsal confocal image of 7mm Tg(-5.2lyve1b:DsRed)nz101 zebrafish larvae following injection of FITC-labeled high molecular weight (2000kDa) dextran. Arrowheads indicate the presence of the dye in lymphatic vessels, but the dye is absent from muLECs. Images representative of data obtained from injection of n=6 larvae. Scale bar represents 100μm.
(b) Magnification of boxed region in (a) showing muLECs do not absorb high MW dextran. Images representative of data obtained from injection of n=6 larvae. Scale bar represents 100μm.
(c) Dorsal confocal image of a Tg(-5.2lyve1b:DsRed)nz101; Tg(fli1a:EGFP)y1 zebrafish larvae. Image representative of n=5 larvae. Scale bar represents 100μm.
(d) Magnification of boxed region in (c) showing muLECs co-express lyve1 and fli1a (arrowheads). Image representative of n=6 larvae. Full quantification of marker co-expression can be found in Supplementary Figure 1f. Scale bar represents 100μm.
(e) muLECs are closely associated with blood vessels in Tg(-5.2lyve1b:DsRed)nz101 larvae. Image representative of n=6 larvae. Scale bar represents 100μm.
(f) Confocal image of a cross-section of adult (20mm stage, 3 month old) Tg(-5.2lyve1b:DsRed)nz101; TgBAC(pdgfrβ:EGFP)uq15bh brain showing pdgfrβ expressing pericytes are associated with blood vessels throughout the adult CNS. Scale bar represents 100μm. Full quantification of marker co-expression can be found in Supplementary Figure 1f. Image representative of data obtained from injection of n=3 male and female 20mm zebrafish adults.
