Supplementary Figure 6: Impact of brain state and anesthesia.
From: Cortical gamma band synchronization through somatostatin interneurons
a) Photo-stimulation of PV neurons in V1 of awake (black, n = 6 mice, same curve as Fig 4b), V1 of isoflurane-anesthetized (gray, n = 4 mice) and S1 of isoflurane anesthetized (blue, n = 3 mice) PV-Cre mice. Anesthesia shifts the peak resonant frequency (p<0.001, significant effect of anesthesia on fold increase (F = 10.01, d.f. = 1) and p<0.001 frequency-anesthesia interaction (F = 7.54, d.f. = 10), 2-way-ANOVA) but the curves for the different brain regions both peak at 48Hz (p=0.44, for effect of brain region on fold increase, 2-way-ANOVA, F = 0.6, d.f. = 1). Error bars denote s.e.m. b) Scatter plot of the absolute peak gamma power for optogenetic suppression of SOM and PV neurons in awake, but non-running conditions (SOM n = 11 mice, p < 0.001; PV: n = 13 mice, p = 0.001, Wilcoxon signed rank test). c) Scatter plot of the fold change of optogenetic suppression for SOM and PV mice comparing running and quiescence conditions.
