Supplementary Figure 2: NeuroTrace-labeled pericytes are embedded in the basal lamina. | Nature Neuroscience

Supplementary Figure 2: NeuroTrace-labeled pericytes are embedded in the basal lamina.

From: A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging

Supplementary Figure 2

(a) Confocal fluorescence images captured from the cortex of Pdgfrb-cre:Tomato transgenic mice stained with collagen IV antibody showing detection of the double layer of the basal lamina completely surrounding capillary pericyte previously labeled with NeuroTrace dye. Single pericytes labeled with NeuroTrace and Tomato were imaged immediately after tissue sectioning but before immunostaining and then reimaged after immunostaining for collagen IV confirming that NeuroTrace labels capillary pericytes (n=11 cells). Top panel is the same cell shown in Figure 4c top only a single z plane. All images are single z-plane.

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