Supplementary Figure 5: Representative images of the CeA and ovBNST of mice with intact or inactive CGRPPBN neurons.
From: Cancer-induced anorexia and malaise are mediated by CGRP neurons in the parabrachial nucleus
a, Fos immunoreactivity and GFP fiber terminal labeling in the CeA of a tumor-bearing TetTox mouse with TetTox:GFP infection of CGRPPBN neurons. Sections are arranged caudal to rostral (top to bottom; bregma -2.055 to -1.055 mm), separated by approximately 60 μm between each section. This mouse was euthanized 21 days after tumor implantation, and food intake was 4.7 g and 5.4 g in final two days before euthanasia. b, Fos immunoreactivity and GFP fiber terminal labeling in the CeA of a GFP control mouse 21 days after tumor implantation. Food intake for this mouse was 1.6 g and 0.0 g in final two days prior to euthanasia. c, Fos immunoreactivity and GFP fiber terminal labeling in the caudal to rostral ovBNST (top to bottom; bregma 0.145 to 0.245 mm) of a tumor-bearing TetTox mouse. d, Fos immunoreactivity and GFP fiber terminal labeling in the ovBNST of a GFP mouse. Scale bars are 100 μm. Related to Fig. 4.
