Supplementary Figure 7: Inactivation of CGRP^PBN neurons prevents cancer-induced weight loss in Apc^min/+ mice and activation of the CeA and ovBNST.

a-c, Quantification and representative histological examples of Fos expression in the CeA and ovBNST of wild-type or tumor-bearing mice with intact (GFP) or inactivated (TetTox) CGRP^PBN neurons (GFP Apc^min/+, n = 7; TetTox Apc^min/+, n = 7; GFP WT, n = 5; CeA, one-way ANOVA: Interaction, F(2, 18) = 30.62, p < 0.0001; ovBNST, one-way ANOVA: Interaction, F(2, 18) = 17.84, p < 0.0001). d, Change in body weight (relative to week 12) of male Apc^min/+ mice (and cancer-free littermates) with intact (GFP) or inactivated (TetTox) CGRP^PBN neurons (GFP Apc^min/+, n = 7; TetTox Apc^min/+, n = 7; GFP WT, n = 5; two-way ANOVA: Interaction, F(8, 76) = 7.10, p < 0.0001; Time, F(4, 76) = 2.85, p = 0.0296; Treatment, F(2, 76) = 77.26, p < 0.0001). e, Change in bodyweight (relative to week 12) of female experimental cohort (GFP Apc^min/+, n = 12; TetTox Apc^min/+, n = 7; GFP WT, n = 4; two-way ANOVA for weeks 12-21: Interaction, F(16, 149) = 3.88, p < 0.0001; Time, F(8, 149) = 1.63, p = 0.1210; Treatment, F(2, 149) = 72.15, p < 0.0001). f, Average daily food intake of the female experimental cohort (two-way ANOVA for weeks 12-21: Interaction, F(18, 168) = 0.90, p = 0.5807; Time, F(9, 168) = 0.58, p = 0.8149; Treatment, F(2, 168) = 13.98, p < 0.0001). † symbols represent euthanasia of GFP Apc^min/+ mouse. # symbols represent euthanasia of TetTox Apc^min/+ mouse. g, Food intake during 24-h period prior to euthanasia (female cohort; two-tailed student’s t-test: t(16) = 13.98, p < 0.0001). Line graphs show mean ± SEM. Box plots show mean (+), median, quartiles (boxes) and range (whiskers) for each treatment. * P ˂ 0.05, ** P ˂ 0.01, *** P ˂ 0.001. Scale bar is 100 μm. Related to Fig. 5.