Supplementary Figure 9: Lepr-Cre-mediated deletion of Cadm1 impacts insulin sensitivity.

(a) Glucose measurements during an ITT on 12-week old Agrp-Cre, Cadm1^flox/flox mice (n=3) and control littermates (n=5). (b) Glucose measurements during an ITT on 12-week old Pomc-Cre, Cadm1^flox/flox mice (n=6) and control littermates (n=6). (c) Glucose measurements during an ITT on 12-week old Sim1-Cre, Cadm1^flox/flox mice (n=6) and control littermates (n=10). (d) Body weight in Lepr-Cre, Cadm1^flox/flox mice (n=4) and littermate controls (n=7). (e) Glucose measurements during an ITT on 12-week old Lepr-Cre, Cadm1^flox/flox mice (n=3) and control littermates (n=5). (f) Correlation of energy expenditure of individual animals plotted against lean body mass in in 12-week old male Lepr-Cre, Cadm1^flox/flox mice (n=7) and littermate controls (n=8). (g) Quantification of locomotor activity and daily food intake in 12-week old Lepr-Cre, Cadm1^flox/flox mice (n=12) and littermate controls (n=12). (h) Body weight in ob/Lepr-Cre, Cadm1^flox/flox mice (n=3) and Lep^ob/ob littermates (n=3). (i) Random-fed and fasted blood glucose and random-fed plasma insulin in 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=5) and Lep^ob/ob littermates (n=7). (j) Glucose measurements during an insulin tolerance test on 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=5) and Lep^ob/ob littermates (n=7). (k) Glucose measurements during a glucose tolerance test on 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=5) and Lep^ob/ob littermates (n=6). (l) Glucose measurements during a pyruvate tolerance test on 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=3) and Lep^ob/ob littermates (n=4). (m) Pancreatic β-cell mass and insulin content measurements in Lepr-Cre, Cadm1^flox/flox (n=5), ob/Lepr-Cre, Cadm1^flox/flox (n=7), Lep^ob/ob (n=5), and wild-type (WT) littermate controls (n=6) from 12 weeks of age. (n) Energy expenditure per individual animals plotted against lean body mass in 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=6) and Lep^ob/ob littermates (n=7). (o) Quantification of locomotor activity and daily food intake in 12-week old ob/Lepr-Cre, Cadm1^flox/flox mice (n=6) and Lep^ob/ob littermates. (n=8). Results in panels a-e, h, and j-l are presented as mean ± s.e.m. *P<0.05, and **P<0.01. Boxplots show median, lower and upper quartiles (box), and minimum and maximum (whiskers). Statistical analyses are described in the Methods and Supplementary Table 3.