Supplementary Figure 2: Increased Cadm1 expression in multiple brain regions of Lepob/ob mice is reversed upon ketogenic diet feeding.
From: Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1
(a) Schematic representation of Cadm1 and Cadm2 engaged in homophilic (homo) and heterophilic (het) interactions. (b) (c) Western blot analysis of Cadm1 and Cadm2 in total and synaptosome-enriched lysates from cerebellum and hippocampus of 4-week-old Lepob/ob mice and littermate controls. (d) Quantification of western blot analysis of Cadm1 in multiple brain regions of 16-week-old WT and Lepob/ob mice on chow or ketogenic diet for 45 days. Cortex (ctx), striatum (str), hippocampus (hpc), hypothalamus (hyp), midbrain (mid), hindbrain (hind), and cerebellum (cer) are shown. (e, f) Western blot analysis of Cadm1 in cerebellum and hippocampus of 16-week-old wild-type on normal chow (WT/chow) and Lepob/ob mice on normal chow (Lepob/ob/chow) or ketogenic diet (Lepob/ob/keto). (g) Western blot analysis of Cadm1 from isolated hippocampus of 12-week old C57Bl/6 mice after administration of ketogenic diet for 4 or 8 days. Results are presented as mean ± s.e.m. *P<0.05 and **P<0.01. Boxplots show median, lower and upper quartiles (box), and minimum and maximum (whiskers). Statistical analyses are described in the Methods and Supplementary Table 3.
