Supplementary Figure 2: Chd8^+/del5 mice from the replication cohort exhibit cognitive deficits, but no ASD social or repetitive phenotypes.

Chd8^+/del5 mice exhibit deficits in learning and memory, including reduced freezing after fear conditioning in both context (A; t_{(1, 40)} = 1.6607, P = 0.1046) and cued assays (B; t_{(1, 40)} = 1.9593, P = 0.0571). Novel Object: Chd8^+/del5 mice fail to show significant difference in exploration between a novel and familiarized object (C; WT F_{(1, 20)} = 4.5583, P = 0.0453; Chd8^+/del5 F_{(1, 19)} = 0.7921, P = 0.3846). Social Approach: Chd8^+/del5 mice do not exhibit differences relative to WT littermates in time spent in chamber with novel mouse (D; WT: F_{(1, 20)} = 10.438, P = 0.0042; Chd8^+/del5: F_{(1, 20)} = 15.470, P = 0.0008), time sniffing a novel mouse (E; WT: F_{(1, 20)} = 20.3750, P = 0.0002; Chd8^+/del5: F_{(1, 20)} = 30.3946, P = 0.00002), or chamber entries (F; F_{(1, 40)} = 0.307, P = 0.583). Male-Female Social Interactions: Chd8^+/del5 mice exhibit no differences between WT littermates in time spent engaged in nose to nose sniffs (G; t_{(1, 20)} = 0.1520, P = 0.8807), nose to anogenital sniffs (H; t_{(1, 20)} = 0.2492, P = 0.8057), time following (I; t_{(1, 20)} = 1.6396, P = 0.1167), or ultrasonic vocalizations (J; t_{(1, 20)} = 0.7010, P = 0.4914) with an estrus female. Repetitive Behavior: Chd8^+/del5 mice do not exhibit any differences in time spent self-grooming (K; t_{(1, 40)} = 0.2489, P = 0.8047). Open Field: Chd8^+/del5 mice do not exhibit any differences in distance traveled (L; t_{(1, 40)} = 0.9799, P = 0.3328). All data shown from 2^nd cohort. Male mice were used in G-J; males and females were used in all other panels. Unpaired t-tests used for A-B, G-L; within genotype repeated measures ANOVA used for C-E; one-way ANOVA used for F. Error bars represent mean ± s.e.m.