Supplementary Figure 4: qRT-PCR validation of differential mRNA expression at P0. | Nature Neuroscience

Supplementary Figure 4: qRT-PCR validation of differential mRNA expression at P0.

From: Germline Chd8 haploinsufficiency alters brain development in mouse

Supplementary Figure 4

A-J. Data from 7 WT and 5 Chd8+/del5 (HT) P0 forebrains (with the exception of G, which used 7 Chd8+/del5 P0 forebrains), with reactions performed in triplicate. For each target, the one highest and one lowest values were removed from analysis to reduce noise. P-values from Student’s t-test. Primers reported in Table S4. A-J WT n: 6, 4, 6, 6, 6, 6, 7, 6, 6, 6; Chd8+/del5 n: 7, 4, 5, 5, 4, 5, 7, 5, 5, 4.

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