Supplementary Figure 6: P0 and P7 coronal sections reveal no obvious perturbations to cortical lamination.
From: Germline Chd8 haploinsufficiency alters brain development in mouse
Single optical sections of wild type (A-D, I-M) and Chd8+/del5 (E-H, N-R) brains were imaged at 4X and paired based on corresponding anatomical depth of section. Immunofluorescence corresponding to Ctip2 (green; B, F, J, O), Tbr1 (red; C, G, K, P), and Brn2 (magenta; L, Q) was collected via sequential imaging and merged with DAPI (blue; D, H, M, R). Scale bars in E and N are 500 μm. P0: Tbr1 thickness P = 0.34, Ctip2 thickness P = 0.81, P7: Tbr1 thickness P = 0.88, Ctip2 thickness P = 0.69, Brn2 thickness P = 0.32. P-values derived from Student’s t-test. P0: WT n = 8, Chd8+/del5 n = 10; P7: WT n = 7, Chd8+/del5 n = 10.
