Supplementary Figure 7: Changes in splicing in Chd8+/del5 mice.
From: Germline Chd8 haploinsufficiency alters brain development in mouse
A. DE down-regulated genes are enriched in DS genes, but not up-regulated DE genes. DS genes from our data overlap significantly with DS genes identified in a recent postmortem ASD cortex study25. B. (Left) Genome Browser representation of the alternatively spliced Srsf7 intron (chr17:80,604,601-80,603,728). (Right) Isoform-specific qRT-PCR analysis of the Srsf7 intron across development in WT and e17.5 Chd8+/del5 (HT) mice. n: e12.5 = 7, e14.5 = 9, e17.5 Chd8+/del5 = 7, e17.5 WT = 7, P0 = 11. Student’s t-test, e14.5 WT to e17.5 Chd8+/del5, P = 0.30, e17.5 Chd8+/del5 to e17.5 WT, P = 0.62. Linear regression across developmental time points, adjusted R2 = 0.37, P = 1.32E-05. Error bars represent mean ± s.e.m.
