Supplementary Figure 1: Raw western blots.
From: Germline Chd8 haploinsufficiency alters brain development in mouse
Western blots of Chd8 and Gapdh protein in adult 5-bp del and 14-bp del Chd8 mutant lines show decreased protein levels in Chd8+/del5 mice from both alleles. WT n = 3 for 280 kDa, WT n = 4 for 110 kDa, 5-bp n = 3, 14-bp n = 3. There is a significant difference in Chd8 protein abundance in Chd8+/del5 mice at 280 kDa (ANOVA *P = 0.0118, 5-bp *P = 0.0319; 14-bp *P = 0.0328) but not at 110 kDa (ANOVA P = 0.1676, 5-bp P = 0.1086, 14-bp P = 0.4509). B. Chd8 and Gapdh protein expression in WT and Chd8+/del5 (HT) mice at P0. There is a significant difference in Chd8 protein abundance in Chd8+/del5 mice at 280 kDa (n: WT = 9, Chd8+/del5 = 9; *P = 0.0089) and 110 kDa (**P = 0.001). C. Chd8 and Gapdh protein expression in Chd8+/del5 (HT) and WT mice at E14.5. There is a significant difference in Chd8 protein abundance in Chd8+/del5 mice at 280 kDa (n: WT = 6, Chd8+/del5 = 6; *P = 0.02) but not at 110 kDa (P = 0.9903). D. Raw Hnrnpa2b1 western blot from P0 forebrain. One-way ANOVA followed by unpaired post hoc t-tests used in A. Unpaired t-tests used for B-C. Error bars represent mean ± s.e.m.
