Supplementary Figure 3: Representative immunohistochemistry in the cortex and striatum after transduction with AAV-PHP.B or AAV-PHP.eB. | Nature Neuroscience

Supplementary Figure 3: Representative immunohistochemistry in the cortex and striatum after transduction with AAV-PHP.B or AAV-PHP.eB.

From: Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems

Supplementary Figure 3

Single-plane confocal images of native GFP fluorescence (green) after immunohistochemistry (IHC) with DAPI (DNA labeling, blue), NeuN (neuronal marker, magenta) and S100 (glial marker, red) in (a) cortex or (b) striatum. These data are presented in support of Fig. 2. All scale bars are 50 μm. All imaging and display conditions for the GFP channel are matched across panel pairs to allow for comparisons. All images are from tissue extracted at three weeks post intravenous administration of ssAAV-CAG-NLS-GFP at 1 × 1011 vg to adult mice.

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