Supplementary Figure 5: Oral antibiotic treatment does not change major immune cell populations in peripheral blood.
From: Gut microbiota is critical for the induction of chemotherapy-induced pain
a) Body weight comparison between control (H2O) and antibiotic treatment (abx) groups (N=8 each group). There was no significant difference between the two groups by two-way ANOVA test. However, body weight of the abx group was significantly lower than that of the H2O group at day 7 (p=0.03). This difference did not exist at day 14 and day 21. b) Peripheral blood major immune cell population characterization. Peripheral blood samples were stained for T cells (CD3), B cells (B220), dendritic cells (CD11c), monocytes (CD11b+ Ly6G-), neutrophils (CD11+Ly6G+), NK cells (CD335). Samples were acquired with LSR II flow cytometer followed by data analysis using Flowjo software. Abx: antibiotic treatment group; H2O: control group on regular water. Shown dot plots were gated on white blood cells by dumping red blood cells and dead cells based on FSC, SSC parameters. c) Summary of peripheral cell major immune cell populations in antibiotic treatment group (abx) and regular water control group (H2O). Percentages of immune cell populations include T cells, B cells, dendritic cells, monocytes, neutrophils, and NK cells did not differ between the two groups. NS: non-significant.
