Supplementary Figure 6: Apoptosis in primary macrophage cultures.
From: Gut microbiota is critical for the induction of chemotherapy-induced pain
Macrophages were collected from mice peritoneal cavity and were plated on 24-well plate in the presence of indicated concentrations of LPS and oxaliplatin. a) At the beginning of the culture, and 24 hours post-stimulation, cells were harvested for assessment of apoptosis with flow cytometry staining using Annexin V and 7-AAD. Cells positive for Annexin V are in apoptosis, whereas cells positive for both Annexin V and 7-AAD are in late apoptosis/necrosis. At 0 h, there were only small percentages of cells in apoptosis in all treatment groups. The percentages of apoptotic cells (Annexin V positive, Annexin V and 7-AAD double positive) increased at 24 h. b) There was no significant difference among the groups, indicating that the concentrations of oxaliplatin and LPS used in the experiment were not cytotoxic.
