Supplementary Figure 4: Characterization of AAV vector
From: Antipsychotic-induced Hdac2 transcription via NF-κB leads to synaptic and cognitive side effects
(a) Schematic representation of the recombinant AAV vector used to over-express eYFP in cortical pyramidal neurons. (b) Representative image of AAV-mediated transgene expression in mouse frontal cortex. AAV-CaMKIIα::eYFP (AAV-eYFP) was injected into the frontal cortex, and eYFP expression was revealed by immunohistochemistry 3 weeks after surgery. (c,d) Viral-mediated over-expression of eYFP in frontal cortex CaMKIIα-positive (c), but not PV-positive (d), neurons. (e,f) Synaptophysin immunoreactive signal on frontal cortex of mice injected with AAV-eYFP or mock (n = 5 mice per experimental condition). Mean ± s.e.m. n.s., not significant. Two-tailed unpaired t-test (f, P = 0.26, t8 = 0.216). Nuclei were stained in blue with DAPI (c-e). Scale bars, 100 μm (b), 20 μm (c), 50 μm (d,e).
