Supplementary Figure 5: Setup of the novel-object recognition test, and findings in heterozygous Hdac2loxP/+:CaMKIIα-Cre mice | Nature Neuroscience

Supplementary Figure 5: Setup of the novel-object recognition test, and findings in heterozygous Hdac2loxP/+:CaMKIIα-Cre mice

From: Antipsychotic-induced Hdac2 transcription via NF-κB leads to synaptic and cognitive side effects

Supplementary Figure 5

(a) Experimental setup of the novel object recognition test. (b,c) Timeline of the experimental strategy (see Fig. 2f-i). (d) Effect of chronic (21 days) clozapine (10 mg/kg) treatment on exploratory preference in heterozygous HDAC2loxP/+:CaMKIIα-Cre mice and controls (HDAC2loxP/+). Note that chronic clozapine treatment impaired novel object recognition in control (HDAC2loxP/+) mice (n = 9 mice per experimental condition), an effect that did not occur in heterozygous HDAC2loxP/+:CaMKIIα-Cre littermates (n = 7 mice per experimental condition). Mean ± s.e.m. ***P < 0.001; n.s., not significant. Two-way ANOVA with Bonferroni’s post-hoc test (c, controls: P = 0.034, F1,32 = 4.90; heterozygous: P < 0.0002, F1,24 = 26.66).

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