Supplementary Figure 5: Supporting data from in vivo Ca²⁺ imaging experiments from CeA^Htr2a neurons.

a, Cre-dependent GCaMP6s expression in the CeA of an Htr2a-Cre;tdTomato mouse (representative of 3 mice). b, Activity of CeA^Htr2a::GCaMP6s neurons increased during eating compared to baseline period (n = 69 cells across 4 mice, two-tailed unpaired t test: t₍₆₈₎ = 2.52, P = 0.0141). Connected points are responses of individual neurons. c, Histogram of preference indices of individual neurons for feeding during all food eating bouts. d, Example cells from two mice that reduced activity during eating. Grey bars= eating bouts. e, Mean Ca²⁺ responses of cells classified as Down cells (n = 9/69 cells) before and upon eating onset of all recorded feeding bouts. f, Mean Ca²⁺ activity of cells that reduced activity during feeding compared to baseline (n = 9 cells across 4 mice, two-tailed unpaired t test: t₍₈₎ = 3.91 P = 0.0045). Connected points are the average responses of all classified cells during individual bouts. g, Ca²⁺ responses of representative cells averaged across 12 FR1 trials (Upper) (total n = 36 cells from 3 mice). Lower= Ca²⁺ responses of the individual trials. h, Population Ca²⁺ responses of all recorded neurons (n = 36 cells from 3 mice) aligned to eating bout end. Upper = mean. Lower = heat map of normalized Ca²⁺ responses from all neurons. Each row is one trial.

Bar graph values are mean ± SEM. * P < 0.05, ** P < 0.01. Scale bar: 50 μm.