Supplementary Figure 10: Long-range monosynaptic inputs to CeAHtr2a and CeAPKCδ neurons.
From: Central amygdala circuits modulate food consumption through a positive-valence mechanism
a, Numbers of PKCδ, Htr2a, PBN-projecting and PBN-projecting Htr2a starter neurons in CeL and CeM per mouse quantified in Fig.7a-b and panels c-e below. Each dot is a separate experiment. b, Relationship between numbers of starter cells in CeA and number of long range input neurons. Each dot is a separate experiment. c, Proportion of monosynaptic inputs to CeAHtr2a (n = 6 mice), PBN-projecting CeAHtr2a (n = 4 mice), PBN-projecting CeA (n = 5 mice) and CeAPKCδ (n =5 mice) neurons, normalized against the total number of inputs cells in each animal. IC, insula cortex; Isocx, isocortex; Pir, piriform cortex; LA, lateral amygdala; BLA, basolateral amygdala, anterior part; BLP, basolateral amygdala, posterior part; Hipp, hippocampal formation; Enth, enthorinal cortex; BNST, bed nucleus of the stria terminalis; VP, ventral pallidum; aPVT, anterior paraventricular thalamus; pPVT, posterior paraventricular thalamus; Multimod. T., multimodal thalamus; VPMpc, ventral posteromedial nucleus of the thalamus, parvicellular part; PO H., preoptic hypothalamus; PVH, paraventricular hypothalamus; Tub. p. H., tuberal posterior hypothalamus; Post. H., posterior hypothalamus; PSTN, parasubthalamic nucleus; SNL, substantia nigra, lateral part; MRN, midbrain reticular nucleus; CUN, cuneiform nucleus; RR, midbrain reticular nucleus, retrorubral area; DR, dorsal raphe; lPBN, lateral parabrachial nucleus. d, Coefficient of variation for each region giving more than 1% of the total inputs to CeAHtr2a and, or CeAPKCδ neurons. e, Example graphs showing a strong positive correlation between inputs from the IC and VPMpc, PSTN and Tub. p. H. and no correlation between inputs from the Tub.p.H. and IC. Each dot is a separate experiment. Values shown are Pearson correlation coefficients with p values from a two-tailed unpaired t test: VPMPc vs IC: r(4) = 0.98, P = 0.0006; Tub. p. H. vs PSTN: r(4) = 0.83, P = 0.0418; IC vs Tub. p. H.: r(4) = 0.30, P = 0.5678.
Bar graph values are mean ± SEM. Box–whisker plots display median, interquartile range and fifth to ninety-fifth percentiles of the distribution. * P < 0.05, *** P <0.001.
