Supplementary Figure 6: Further characterization of α-synuclein and dopamine derived from A53T mice.
From: Dopamine induces soluble α-synuclein oligomers and nigrostriatal degeneration
(a-b) Triton-soluble SN from A53T TH-RREE and A53T CtrlVect mice at 5 mpi was fractionated by size exclusion chromatography, and the resulting fractions were analyzed by western blot using α-synuclein antibodies Syn211 (C-terminal) (a) and SNL-4 (N-terminal) (b). Oligomer bands that uniquely appear in 122 Å fractions from TH-RREE mice are indicated by arrows. Neural specific enolase (NSE) was used as a loading control, and appears for Syn211 and SNL-4 as the same blot since the same membrane is shown. (n = 4 mice per group). (c) The SDS-PAGE gels were scanned for near-infrared fluorescence, which revealed oxidized dopamine (arrow) only in 72-122 Å fractions from A53T TH-RREE mice. (n = 4 mice per group).
