Supplementary Figure 5: Retrograde tracer labeling.
From: Modular organization of the brainstem noradrenaline system coordinates opposing learning states
(a) Example of different populations of LA/B- (green) and IL- (blue) projecting LC neurons following injection of retrograde tracer (CTb-647) into IL and RVG-GFP into LA/B (n=3 animals). (b) Same as a except LA/B projecting cells are green (from retrograde CAV2-cre-GFP injections) and IL projecting cells are labeled red (CAV2-DsRed into IL) (n=3 animals). TH is pseudocolored in blue. (c) Example of control experiment in which green and red retrobeads were coinjected into IL resulting in a high degree of overlap in LC neurons (green+red/green=80.1%, green+red/red=81.5%) (n=3 animals). Yellow triangles denote double labeled cells. (d) Example of similar non-overlapping noradrenaline populations in mouse LC. Green and red retrobeads were injected into IL and LA/B respectively. In mice, 9.9% of LA/B projecting neurons overlapped with IL projecting, 4.9% overlap of IL projecting cells with LA/B projecting (n=3 animals). TH is pseudocolored blue. All scale bars=20 μm.
