Supplementary Figure 9: Efferent mapping of LA/B- and IL-projecting terminal fields.
From: Modular organization of the brainstem noradrenaline system coordinates opposing learning states
(a) Quantification of the pixel density of axon terminal labeling in each area (pixel count in each region/pixel count in total region) for LA/B (red) and IL (green) projecting cells. (b) Density of innervation of LA/B, IL, ACx1 and ACx2 from TH+, LA/B projecting and IL projecting LC neurons. For a and b, parentheses show number of animals used in each group. (c) Confocal images showing examples of synaptophysin-mCherry labeling of axon terminals in IL, LA/B and ACx from TH-cre expressing, LA/B projecting and IL projecting cell populations. PL, prelimbic; ACC, anterior cingulate; OFC, orbitofrontal cortex; ACx, auditory cortex; CeA/MeA, central and medial nuclei of the amygdala; IC, insula; PRh, perirhinal; BNST, bed nucleus of the stria terminalis; dHip/vHip, dorsal/ventral hippocampus; Hpt, hypothalamus; MT, medial thalamus; VPM/L, ventroposterior medial/lateral thalamus; MgM, medial geniculate thalamus; VTA, ventral tegmental area; SN, substantia nigra; v/dPAG, ventral/dorsal periaqueductal gray. For a&b, individual data-points are represented as black dots. Data represent the mean ± SEM, and the numbers in parentheses represent the number of samples. Scale bar=20 μm.
