Figure 2: GLUK5 antagonists and pilocarpine-induced epileptiform activity.
From: Antagonists of GLUK5-containing kainate receptors prevent pilocarpine-induced limbic seizures
(a) Reduction of epileptiform activity in vitro by LY377770. Electrically evoked synaptic responses (top) and DC recordings (middle) in control conditions (i), following the addition of 15 μM pilocarpine (ii), following the addition of 1.5 μM LY377770 to the pilocarpine-containing medium (iii) and following washout of LY377770 and pilocarpine (iv). The synaptic responses are averages of four successive responses that were obtained at the times indicated on the graph (i–iv). The DC recordings are 10-min excerpts to illustrate the maximum effects of the different treatments. The regular deflections in the excerpts of the chart records are stimulus artifacts, upon which are superimposed the pilocarpine-induced spontaneous inter-ictal bursts. Bottom, electrode placements for recording spontaneous and electrically evoked responses in area CA3 of the rat hippocampal slice. (b) Prevention of the initiation of epileptiform activity in vitro by LY377770. These are equivalent electrically evoked synaptic responses, but 1.5 μM LY377770 was added before 15 μM pilocarpine. LY377770 prevented the development of epileptiform activity, as determined 2 h after washout of LY377770 (v), but at this time, a second application of pilocarpine readily induced epileptiform activity (vi).
