Figure 3: The induction of epileptiform activity requires activation of kainate but not AMPA receptors.
From: Antagonists of GLUK5-containing kainate receptors prevent pilocarpine-induced limbic seizures
Data are presented as in Fig. 2b. (a) The selective GLUK5 receptor antagonist, LY382884 (10 μM), did not affect low-frequency synaptic responses (ii), but prevented the development of pilocarpine-induced epileptiform activity (iii–v). A second application of pilocarpine readily induced epileptiform activity 2.5 h after washout of LY382884 (vi). (b) The AMPA receptor antagonist, GYKI53655 (30 μM), substantially antagonized the low-frequency synaptic response before, during and immediately after (ii) co-perfusion of 15 μM pilocarpine. After washout of GYKI53655, epileptiform activity appeared, although pilocarpine was not re-applied (iii–iv). This epileptiform activity was reversibly blocked by LY382884 (10 μM; v and vi). The graph above, on the same time scale, shows the time course of washout of 30 μM GYKI53655 monitored using recordings of mossy fiber–evoked fEPSPs. Note that full recovery takes approximately 2 h.
