Figure 3

Metformin improves nuclear shape organization in HGPS MSCs. (a) Typical nuclear shape organization observed in HGPS MSCs stained for lamin A/C. (b) Lamin A/C immunostaining in HGPS MSCs following 48 h of treatment with 5 mmol/l metformin and 1 μmol/l tipifarnib (FTI). (c) Quantification of nuclear shape abnormalities following 48 h of treatment with 5 mmol/l metformin and 1 μmol/l tipifarnib (FTI). Statistical analysis was performed by one-way analysis of variance (ANOVA), using Dunnett’s comparison test. Values of P<0.05 were considered significant (***P<0.001). HGPS, Hutchinson–Gilford progeria syndrome; FTI, farnesyl transferase inhibitor; Met, metformin; MSC, mesenchymal stem cell.