Figure 4

Simulated spatial patterns of specific growth rate in a 2D heterogeneous biofilm structure compared with an in vitro result. (a) Acridine orange stained frozen section showing regions of relatively rapid (red/orange) and slow (green/yellow) growth (reprinted with permission from ref. 30). (b) Calculated glucose concentration (mg l⁻¹) for a bulk fluid glucose concentration of 30 mg l⁻¹. (c–e) The predicted specific growth rate (h⁻¹) for bulk fluid glucose concentrations of 10 mg l⁻¹, (c); 25 mg l⁻¹, (d); and 40 mg l⁻¹, (e). Bar=100 μm.