Figure 1 | npj Biofilms and Microbiomes

Figure 1

From: Real-time optotracing of curli and cellulose in live Salmonella biofilms using luminescent oligothiophenes

Figure 1

LCO staining patterns distinguish Salmonella biofilms. (a) Structure of h-HTAA and h-FTAA. (b) Schematic of the incline glass coverslip setup enabling microscopic analysis of biofilm at air–liquid interface after removal of coverslips. (c–f) Fluorescence confocal microscopy using indicated excitation and emission wavelengths (left) and transmission confocal microscopy (right) of h-HTAA- and h-FTAA-stained biofilms from strains 3934 (c) wt, (d) ΔcsgD, (e) ΔbcsA and (f) ΔcsgA with indicated curli and cellulose phenotypes. Single optical sections are shown. Scale bar=50 μm.

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