Figure 2
LCO-based fluorometric biofilm quantification in a small-volume 96-well assay. (a) Growth curve, shown as viable counts, of strain 3934 wt cultured in the absence (control) and presence of h-HTAA and h-FTAA. (b and c) End point quantification of biofilm formed by 3934 wt at indicated times based on (b) fluorescence from cultures grown in the presence of h-HTAA and h-FTAA and (c) the crystal violet assay. (d–f) Quantification of biofilm formed at 24 and 48 h by 3934 wt (■), ΔbcsA (), ΔcsgA (▨) and ΔcsgD (□) based on fluorescence from (d) h-HTAA, (e) h-FTAA, and based on the (f) crystal violet assay. Data represent n: 1 of 3 with standard deviations shown. CFU, colony forming units; CV, crystal violet; RFU, relative fluorescence units.
